Enzymatic Study of Linoleic and Alpha-Linolenic Acids Biohydrogenation by Chloramphenicol-Treated Mixed Rumen Bacterial Species
نویسندگان
چکیده
منابع مشابه
Fatty acids in forages. II. In vitro ruminal biohydrogenation of linolenic and linoleic acids from timothy
1Département des sciences animales, Faculté des sciences de l’agriculture et de l’alimentation, Université Laval, Sainte-Foy, Québec, Canada G1K 7P4; 2Agriculture and Agri-Food Canada, Soils and Crops Research and Development Centre, Sainte-Foy, Quebec, Canada G1V 2J3 (e-mail: [email protected]); and 3Agriculture and Agri-Food Canada, Dairy and Swine Research and Development Centre, Lennoxvi...
متن کاملThe biohydrogenation of alpha-linolenic acid and oleic acid by rumen micro-organisms.
1. alpha-[U-(14)C]Linolenic acid was incubated with the rumen contents of sheep and the metabolic products were characterized by thin-layer chromatography, gas-liquid chromatography and absorption spectroscopy in the ultraviolet and infrared. 2. A tentative scheme for the biohydrogenation route to stearic acid is presented. The main pathway is through diconjugated cis-cis-cis-octadecatrienoic a...
متن کاملBiohydrogenation of Unsaturated Fatty Acids by Rumen Bacteria.
Polan, C. E. (North Carolina State of the University of North Carolina, Raleigh), J. J. McNeill, and S. B. Tove. Biohydrogenation of unsaturated fatty acids by rumen bacteria. J. Bacteriol. 88:1056-1064. 1964.-A simple, rapid, specific assay for the biohydrogenation of unsaturated fatty acids was developed. With this assay, it was shown that washed suspensions of mixed rumen bacteria hydrogenat...
متن کاملEffects of pH and concentrations of linoleic and linolenic acids on extent and intermediates of ruminal biohydrogenation in vitro.
Three experiments were conducted by in vitro incubations in ruminal fluid to investigate the effects of pH and amounts of linoleic and linolenic acids on the extent of their biohydrogenation, the proportions of conjugated linoleic acid (CLA) and trans-C18:1 as intermediates, and the ratio trans-10:trans-11 intermediates. The effects of pH and amount of linoleic acid were investigated in kinetic...
متن کاملWhole body distribution of deuterated linoleic and alpha-linolenic acids and their metabolites in the rat.
Little is known about the uptake or metabolism of essential fatty acids (EFAs) in various mammalian organs. Thus, the distribution of deuterated alpha-linolenic acid (18:3n-3) and linoleic acid (18:2n-6) and their metabolites was studied using a stable isotope tracer technique. Rats were orally administered a single dose of a mixture (20 mg each) of ethyl D5-18:3n-3 and D5-18:2n-6, and 25 tissu...
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ژورنال
عنوان ژورنال: Frontiers in Microbiology
سال: 2018
ISSN: 1664-302X
DOI: 10.3389/fmicb.2018.01452